An autopsy was conducted on a fossil wolf that lived over 44,000 years ago, found mummified in the Siberian permafrost, at the Mammoth Museum Laboratory of the North-Eastern Federal University.

Researchers took samples of internal organs to identify and study ancient viruses and microbiota, as well as the animal’s diet, analyze its genome, and compare it with its modern relatives. This is the first complete discovery of an adult predator of such geological antiquity, unparalleled in the world.

The well-preserved mummy of the fossil wolf was discovered in 2021 in the permafrost, about 40 meters deep, in the Tirekhtyakh River in the Abyysky district by local residents. For scientific research, the find was delivered to the Academy of Sciences of the Republic of Sakha (Yakutia).

Albert Protopopov, head of the mammoth fauna study department at the Yakutia Academy of Sciences, emphasized that this is the first global find of an adult predator from the Late Pleistocene. Its stomach has been preserved in isolation, without contamination, making the task complex. As a result of the preparation carried out, we expect to obtain a snapshot of the ancient Pleistocene biota. It was an active and large predator, and we have the opportunity to know what it ate. Additionally, its stomach also contains objects consumed by its prey, the scientist commented.

Another view of the Pleistocene wolf found in Siberia.
Another view of the Pleistocene wolf found in Siberia. Credit: Michil YAKOVLEV / M.K.Ammosov North-Eastern Federal University

In sterile conditions, scientists took samples of the internal organs to study the animal’s diet, ancient viruses, and microbiota, as well as samples from the find to compare its genome with that of the modern wolf. Samples were also taken for other studies. Additionally, we took a premolar – a tooth to determine the biological age of the find. But from the wear of the teeth and the development of the sagittal crest, we can already say that it is an adult male, explained Maxim Cheprasov, head of the Mammoth Museum Laboratory at the North-Eastern Federal University. He added that after thorough research of the find, scientists at the North-Eastern Federal University will begin studying another wolf found in the Nizhnekolymsky district of Yakutia, brought in 2023.

Our main goal is to understand what this wolf ate, who it was, and how it relates to the ancient wolves that inhabited the northeastern part of Eurasia, particularly Yakutia, commented Artem Nedoluzhko, scientific director of the paleogenomics laboratory at the European University in St. Petersburg.

He emphasized the importance of continuing collaboration with the university. According to him, only interdisciplinary studies using various methods allow for a deeper understanding of the planet’s history, understanding what caused the mass extinctions of various animal and plant species, and generally predicting the future.

Artemy Goncharov, head of the functional genomics and proteomics laboratory at the Institute of Experimental Medicine, professor in the department of epidemiology, parasitology, and disinfection at the North-Western State Medical University of Russia, added that they have been conducting joint research with scientists from the North-Eastern Federal University for ten years. Our scientific interaction with the Mammoth Museum at the North-Eastern Federal University has allowed us to learn more about the bacteria that inhabited the mammoth steppe since the Pleistocene period. We see that in fossil animal finds, live bacteria can survive for millennia, bearing witness to those ancient times. We hope to obtain good results that will allow us to advance in understanding what ancient microbial communities were like, what role they played, and what dangerous pathogenic bacteria were present in their structure. Possibly, microorganisms will be discovered that can be applied in medicine and biotechnology as producers of biologically active substances. Therefore, these studies have a significant impact on the future, concluded the scientist.



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